重组溶葡萄球菌素，Lysostaphin，Lysostaphin Recombinant

INTRODUCTION

Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination. 

SYNONYMS

Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.

DESCRIPTION

Lysostaphin Recombinant produced in E.Coli is a non-glycosylated polypeptide chain having a molecular mass of 26.92 kDa.

SOURCE

Escherichia Coli.

PHYSICAL APPEARANCE

Sterile Filtered lyophilized powder.

FORMULATION

The protein was lyophilized without any additives.

STABILITY

Lyophilized Lysostaphin although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysostaphin can be stored at 4°C up to 3 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.

SOLUBILITY

It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5 for optimal stability, which can then be further diluted to other aqueous solutions such as 200mM Tris HCL pH-8 for optimal activity. 

PURITY

97% as determined by RP-HPLC.

BIOLOGICAL ACTIVITY

Assessed by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH-8, 30°C.  Lysostaphin is a zinc enzyme therefore EDTA is an inhibitory factor.

SPECIFIC ACTIVITY

Determined to be 3,542 units/mg.

PROTEIN CONTENT

Protein quantitation was carried out by two independent methods 
1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 
2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.

SAFETY DATA SHEET

SDS

USAGE

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.