Recombinant Mouse Leptin，Leptin

产品说明(Description)

Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed.

Accession #: Q544U0

Known as: Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.5.

质量控制(Quality Control)

Purity:            Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:    Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景(Background)

Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence.