Recombinant Human GM-CSF，GM-CSF

产品说明(Description)

Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed. 

Accession #: P04141

Known as: Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:     Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity:    Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

                        The ED50 for this effect is 4-20 pg/mL.                           

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

背景（Background）

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines.

电泳(SDS-PAGE)

参考文献(Reference)

The Natural Compound Notopterol Binds and Targets JAK2/3 to Ameliorate Inflammation and Arthritis[J]. Qiong Wang. et al. Cell Reports. 2020.

HIV-2/SIV Vpx targets a novel functional domain of STING to selectively inhibit cGAS–STING-mediated NF-κB signalling[J]. Jiaming Su. et al. Nature Microbiology. 2019.

A cell‐free expression and purification process for rapid production of protein biologics[J]. Sullivan C J. et al. Biotechnology journal. 2015.

Methods for transferring gene into silkworm and expression of hGM-CSF in silk glands of transgenic silkworm[J]. Li Z. et al. Journal of Chemical and Pharmaceutical Research. 2014.