Recombinant Human PKM2，Pyruvate kinase PKM ，PKM2

产品说明(Description)

Recombinant Human Pyruvate kinase isoform M2  is produced by our E.coli expression system and the target gene encoding Ser2-Pro531 is expressed with a 6His tag at the N-terminus. 

Accession #: P14618

Known as: Pyruvate kinase PKM; CTHBP; OIP-3; THBP1

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 0.05% Brij-35, 1mM DTT, pH7.5.

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

背景(Background)

Pyruvate kinase isoform M2 (PKM2) is a member of the pyruvate kinase (PK) family, a pivotal glycolytic enzyme that is consistently changed during tumorigenesis. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets. As the rate-controlling enzyme in glycolysis, PKM2, has also been found to be expressed in embryonic, proliferating, and tumor cells, and it has been considered to be crucial for the metabolism and growth of tumor cells. Recent studies have shown that PKM2 promotes cell proliferation and suppresses cell apoptosis in various tumors. In addition, it has been reported that the PKM2 knockdown affects the Akt and ERK protein kinases.